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ZADH2 Polyclonal antibody

ZADH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14679-1-AP

Synonyms

ZADH2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZADH2 Polyclonal antibody (14679-1-AP)

    WB analysis of mouse brain using 14679-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14679-1-AP (ZADH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZADH2 Polyclonal antibody (14679-1-AP)

    WB analysis of mouse brain using 14679-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14679-1-AP (ZADH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZADH2 Polyclonal antibody (14679-1-AP)

    IHC staining of human pancreas cancer using 14679-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14679-1-AP (ZADH2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZADH2 Polyclonal antibody (14679-1-AP)

    IHC staining of human pancreas cancer using 14679-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14679-1-AP (ZADH2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14679-1-AP targets ZADH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZADH2 fusion protein Ag6390
Full Name zinc binding alcohol dehydrogenase domain containing 2
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC078661
Gene Symbol ZADH2
Gene ID (NCBI) 284273
RRIDAB_2218521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZADH2 antibody 14679-1-APDownload protocol
IHC protocol for ZADH2 antibody 14679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols