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WIPF2 Polyclonal antibody

WIPF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20512-1-AP

Synonyms

WICH, WIP related protein, WIPF2, WIRE

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using WIPF2 Polyclonal antibody (20512-1-AP)

    WB analysis of mouse brain using 20512-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using WIPF2 Polyclonal antibody (20512-1-AP)

    WB analysis of mouse colon using 20512-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20512-1-AP (WIPF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WIPF2 Polyclonal antibody (20512-1-AP)

    IHC staining of human breast cancer using 20512-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WIPF2 Polyclonal antibody (20512-1-AP)

    IHC staining of human liver cancer using 20512-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20512-1-AP (WIPF2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, mouse colon tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20512-1-AP is WIPF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPF2 fusion protein Ag14330
Full Name WAS/WASL interacting protein family, member 2
Calculated Molecular Weight 440 aa, 46 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC065551
Gene Symbol WIPF2
Gene ID (NCBI) 147179
RRIDAB_10695185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WIPF2 antibody 20512-1-APDownload protocol
IHC protocol for WIPF2 antibody 20512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols