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WBSCR17 Polyclonal antibody

WBSCR17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21280-1-AP

Synonyms

Polypeptide N-acetylgalactosaminyltransferase 17, Polypeptide GalNAc transferase-like protein 3, GALNT17, GalNAc-T-like protein 3, GalNAc T like protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of mouse brain using 21280-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of mouse lung using 21280-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of human brain using 21280-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using WBSCR17 Polyclonal antibody (21280-1-AP)

    WB analysis of A549 using 21280-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21280-1-AP (WBSCR17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human brain using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human brain using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human heart using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using WBSCR17 Polyclonal antibody (21280-1-AP)

    IHC staining of human heart using 21280-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21280-1-AP (WBSCR17 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, mouse lung tissue, human brain tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21280-1-AP targets WBSCR17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WBSCR17 fusion protein Ag15792
Full Name Williams-Beuren syndrome chromosome region 17
Calculated Molecular Weight 598 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC069624
Gene Symbol WBSCR17
Gene ID (NCBI) 64409
RRIDAB_10732815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WBSCR17, also known as GALNT17, which encodes a brain-expressed N-acetylgalactosaminyl transferase (GalNAcT), is located at the distal edge of a region that is commonly deleted or duplicated in Williams Beuren Syndrome (WBS), a developmental disorder with motor and coordination problems, impaired visuospatial memory, and abnormal social interaction (PMID: 31554716). WBSCR17 loss-of-function has significant effects on cerebellar development, and is associated with phenotypes including developmental delay, deficits in motor coordination, reduced exploratory activity, and impaired social behavior (PMID: 22787146).

Protocols

Product Specific Protocols
WB protocol for WBSCR17 antibody 21280-1-APDownload protocol
IHC protocol for WBSCR17 antibody 21280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols