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WASL Polyclonal antibody

WASL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14306-1-AP

Synonyms

N-WASP, NWASP, Neural Wiskott-Aldrich syndrome protein, N WASP, Actin nucleation-promoting factor WASL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using WASL Polyclonal antibody (14306-1-AP)

    WB analysis of mouse brain using 14306-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using WASL Polyclonal antibody (14306-1-AP)

    WB analysis of mouse brain using 14306-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14306-1-AP (WASL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using WASL Polyclonal antibody (14306-1-AP)

    IHC staining of human brain using 14306-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using WASL Polyclonal antibody (14306-1-AP)

    IHC staining of human brain using 14306-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14306-1-AP (WASL antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WASL Polyclonal antibody (14306-1-AP)

    IF Staining of HepG2 using 14306-1-AP

    Immunofluorescent analysis of HepG2 cells, using WASL antibody 14306-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14306-1-AP targets WASL in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WASL fusion protein Ag5424
Full Name Wiskott-Aldrich syndrome-like
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC052955
Gene Symbol WASL
Gene ID (NCBI) 8976
RRIDAB_10638478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WASL antibody 14306-1-APDownload protocol
IHC protocol for WASL antibody 14306-1-APDownload protocol
IF protocol for WASL antibody 14306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Authors - Chunlin Wang
View Article
human,mouseWB,IF,CoIP

iScience

WASH interacts with Ku to regulate DNA double-stranded break repair.

Authors - Tao Wang
View Article
humanWB

J Dermatol Sci

Negative pressure accelerated monolayer keratinocyte healing involves Cdc42 mediated cell podia formation.

Authors - Hsu Chih-Chin CC
View Article
humanWB

Neuroreport

Decreased expression of cortactin in the schizophrenia brain.

Authors - Hriday P Bhambhvani
View Article
humanWB

BMC Cancer

Lymphocyte migration regulation related proteins in urine exosomes may serve as a potential biomarker for lung cancer diagnosis

Authors - Shuai Jin
View Article
humanIHC,WB

Respir Res

Tuftelin1 drives experimental pulmonary fibrosis progression by facilitating stress fiber assembly

Authors - Caoyuan Niu
View Article