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VIPR1 Polyclonal antibody

VIPR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14878-1-AP

Synonyms

HVR1, II, PACAP R 2, PACAP R2, PACAP type II receptor, RDC1, VAPC1, VIP R 1, VIPR, VIPR1, VIRG, VPAC1, VPCAP1R

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    WB analysis of mouse small intestine using 14878-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14878-1-AP (VIPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VIPR1 Polyclonal antibody (14878-1-AP)

    IHC staining of mouse brain using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse small intestine tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14878-1-AP targets VIPR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VIPR1 fusion protein Ag6650
Full Name vasoactive intestinal peptide receptor 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC064424
Gene Symbol VIPR1
Gene ID (NCBI) 7433
RRIDAB_2878089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VIPR1 antibody 14878-1-APDownload protocol
IHC protocol for VIPR1 antibody 14878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

The p65-LOC727924-miR-26a/KPNA3-p65 regulatory loop mediates vasoactive intestinal peptide effects on osteoarthritis chondrocytes

Authors - Yusheng Li
View Article