- Featured Product
- KD/KO Validated
VDAC1/Porin Polyclonal antibody
VDAC1/Porin Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IP, IF, FC, PLA, IHC, CoIP, ELISA
Conjugate
Unconjugated
123
Cat no : 55259-1-AP
Synonyms
"VDAC1/Porin Antibodies" Comparison
View side-by-side comparison of VDAC1/Porin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | RAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver cancer tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55259-1-AP targets VDAC1/Porin in WB, IP, IF, FC, PLA, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, monkey, hamster, pig, canine, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | voltage-dependent anion channel 1 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | NM_003374 |
| Gene Symbol | VDAC1 |
| Gene ID (NCBI) | 7416 |
| RRID | AB_10837225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IHC protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| IF protocol for VDAC1/Porin antibody 55259-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology | ||
Nat Commun An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions. | ||
Nucleic Acids Res The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. | ||
Mol Biol Evol Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans | ||
Redox Biol Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner. | ||
Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (04-22-2021) | good antibody. works very well with mouse cell lines.
 |
