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1A6/DRIM Polyclonal antibody

1A6/DRIM Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18830-1-AP

Synonyms

DRIM, NNP73, Novel nucleolar protein 73, Protein Key 1A6, UTP20

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of HeLa using 18830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of HepG2 using 18830-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    WB analysis of mouse ovary using 18830-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18830-1-AP (1A6/DRIM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IP experiment of HeLa using 18830-1-AP

    IP result of anti-1A6/DRIM (IP:18830-1-AP, 4ug; Detection:18830-1-AP 1:800) with HeLa cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of Hela cells, using UTP20 antibody 18830-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using 1A6/DRIM Polyclonal antibody (18830-1-AP)

    IF Staining of HeLa using 18830-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18830-1-AP (1A6/DRIM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse ovary tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18830-1-AP targets 1A6/DRIM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name UTP20, small subunit (SSU) processome component, homolog (yeast)
Calculated Molecular Weight 318 kDa
Observed Molecular Weight 310 kDa
GenBank Accession NumberNM_014503
Gene Symbol UTP20
Gene ID (NCBI) 27340
RRIDAB_10597541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UTP20, also named as DRIM, interacts with KIAA0649.

Protocols

Product Specific Protocols
WB protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IF protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
IP protocol for 1A6/DRIM antibody 18830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols