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  • Featured Product
  • KD/KO Validated

UBE2G1 Polyclonal antibody

UBE2G1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12012-1-AP

Synonyms

Ubiquitin carrier protein G1, UBE2G, UBC7, EC:2.3.2.23, E217K

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBE2G1 Polyclonal antibody (12012-1-AP)

    WB analysis of HeLa using 12012-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12012-1-AP (UBE2G1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2G1 Polyclonal antibody (12012-1-AP)

    WB analysis of HeLa using 12012-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12012-1-AP (UBE2G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2G1 Polyclonal antibody (12012-1-AP)

    WB analysis of HeLa using 12012-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12012-1-AP (UBE2G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using UBE2G1 Polyclonal antibody (12012-1-AP)

    IHC staining of human stomach using 12012-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12012-1-AP (UBE2G1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using UBE2G1 Polyclonal antibody (12012-1-AP)

    IHC staining of human stomach using 12012-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12012-1-AP (UBE2G1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBE2G1 Polyclonal antibody (12012-1-AP)

    IF Staining of HepG2 using 12012-1-AP

    Immunofluorescent analysis of HepG2 cells, using UBE2G1 antibody 12012-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12012-1-AP targets UBE2G1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2G1 fusion protein Ag2625
Full Name ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast)
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC026288
Gene Symbol UBE2G1
Gene ID (NCBI) 7326
RRIDAB_10665812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2G1 antibody 12012-1-APDownload protocol
IHC protocol for UBE2G1 antibody 12012-1-APDownload protocol
IF protocol for UBE2G1 antibody 12012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Dev

Sequential replication-coupled destruction at G1/S ensures genome stability.

Authors - Kate E Coleman
  • KD Validated
View Article
humanWB

Elife

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation.

Authors - Lu Lv
View Article