beta Tubulin Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 10068-1-AP

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Freight/Packing

Con: 20 μg/150 μl

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Species specificity:
human, mouse, rat, zebrafish

Positive WB detected in:
multi-lysates, Jurkat cells, human brain tissue, HeLa cells , HEK-293 cells, HepG2 cells, SH-SY5Y cells, A431 cells, NIH/3T3 cells, Neuro-2a cells, mouse brain tissue, COLO 320 cells, HeLa cells, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
mouse brain tissue, human brain tissue, mouse brain tissue, rat brain tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
tubulin, beta 3

Calculated molecular weight:
450aa,50 kDa

Observed molecular weight:
50-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TUBB3

Synonyms
beta 4, beta tubulin, MC1R, TUBB, tubulin
Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.


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