alpha Tubulin AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IP, IHC, IF, FC|
|Cited applications||IF, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Cited species||human, mouse, rat|
|Positive WB detected in||multi-cells/tissue, HeLa cells, mouse brain tissue, mouse colon tissue, mouse eye tissue, mouse liver tissue, mouse lung tissue, mouse uterus tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue|
|Positive IF detected in||MCF-7 cells, HeLa cells|
|Positive FC detected in||HEK-293 cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- Western blot on multiple cells with anti-Tubulin-Alpha (11224-1-AP) at dilution 1:1,000.
- Immunofluorescent analysis of MCF-7 cells using 11224-1-AP( Tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- Western blot of Hela cell with anti-Tubulin-Alpha (11224-1-AP) at various dilutions.
- 1X10^6 HEK-293 cells were stained with 0.2ug Tubulin-Alpha antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
- Immunohistochemical of paraffin-embedded human colon cancer using 11224-1-AP(Tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)
- Immunohistochemical of paraffin-embedded human colon cancer using 11224-1-AP(Tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)
- IP Result of anti-Tubulin-Alpha (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||alpha Tubulin fusion protein ag1727||Full name||tubulin, alpha 1b|
|BC009314||Gene ID (NCBI)||10376|
|Gene symbol||TUBA1B||Synonyms||alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, TUBA1B, tubulin, tubulin, alpha 1b|
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.