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TTC39B Polyclonal antibody

TTC39B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23432-1-AP

Synonyms

C9orf52, TPR repeat protein 39B, TTC39B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TTC39B Polyclonal antibody (23432-1-AP)

    WB analysis of mouse testis using 23432-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23432-1-AP (TTC39B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TTC39B Polyclonal antibody (23432-1-AP)

    WB analysis of mouse testis using 23432-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23432-1-AP (TTC39B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTC39B Polyclonal antibody (23432-1-AP)

    IHC staining of human liver cancer using 23432-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23432-1-AP (TTC39B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTC39B Polyclonal antibody (23432-1-AP)

    IHC staining of human liver cancer using 23432-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23432-1-AP (TTC39B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC39B Polyclonal antibody (23432-1-AP)

    IHC staining of human liver using 23432-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23432-1-AP (TTC39B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC39B Polyclonal antibody (23432-1-AP)

    IHC staining of human liver using 23432-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23432-1-AP (TTC39B Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23432-1-AP targets TTC39B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC39B fusion protein Ag20081
Full Name tetratricopeptide repeat domain 39B
Calculated Molecular Weight 682 aa, 77 kDa
Observed Molecular Weight 59 kDa, 67 kDa
GenBank Accession NumberBC142985
Gene Symbol TTC39B
Gene ID (NCBI) 158219
RRIDAB_2879279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TTC39B antibody 23432-1-APDownload protocol
IHC protocol for TTC39B antibody 23432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols