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  • Featured Product
  • KD/KO Validated

TSC22D4 Polyclonal antibody

TSC22D4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 55017-1-AP

Synonyms

THG 1, THG1, TILZ2, Tsc 22 like protein THG 1, TSC22 domain family protein 4, TSC22 domain family, member 4, TSC22D4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TSC22D4 Polyclonal antibody (55017-1-AP)

    IF Staining of HeLa using 55017-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55017-1-AP (TSC22D4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55017-1-AP targets TSC22D4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name TSC22 domain family, member 4
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberNM_030935
Gene Symbol TSC22D4
Gene ID (NCBI) 81628
RRIDAB_10860903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC22D4, also named as TILZ2 and THG-1, is a transcriptional repressor. It is a leucine zipper-containing protein that is highly conserved during evolution. TSC22D4 is transcriptionally up-regulated by many different stimuli, including anti-cancer drugs and growth inhibitors. It plays a suppressive role in tumorigenesis. TSC22D4 interacts with TSC22D1 by forming heterodimers(PMID:10488076).This antibody is specific to TSC22D4.

Protocols

Product Specific Protocols
WB protocol for TSC22D4 antibody 55017-1-APDownload protocol
IF protocol for TSC22D4 antibody 55017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Cell Physiol

The interplay between TGF-β-stimulated TSC22 domain family proteins regulates cell-cycle dynamics in medulloblastoma cells

Authors - Jessica Dragotto
  • KD Validated
View Article