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TSC22D2 Polyclonal antibody

TSC22D2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25418-1-AP

Synonyms

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis using 25418-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis of HepG2 using 25418-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of MDA-MB-453s cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    WB analysis of MDA-MB-453s using 25418-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    IP experiment of HepG2 using 25418-1-AP

    IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human kidney using 25418-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human colon using 25418-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human colon using 25418-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSC22D2 Polyclonal antibody (25418-1-AP)

    IHC staining of human kidney using 25418-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TSC22D2 Polyclonal antibody (25418-1-AP)

    IF Staining of HepG2 using 25418-1-AP

    Immunofluorescent analysis of HepG2 cells using 25418-1-AP (TSC22D2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, HepG2 cells, SH-SY5Y cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25418-1-AP targets TSC22D2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSC22D2 fusion protein Ag22294
Full Name TSC22 domain family, member 2
Calculated Molecular Weight 780 aa, 79 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC044643
Gene Symbol TSC22D2
Gene ID (NCBI) 9819
RRIDAB_2880069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).

Protocols

Product Specific Protocols
WB protocol for TSC22D2 antibody 25418-1-APDownload protocol
IHC protocol for TSC22D2 antibody 25418-1-APDownload protocol
IF protocol for TSC22D2 antibody 25418-1-APDownload protocol
IP protocol for TSC22D2 antibody 25418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

A novel cuproptosis-related gene model predicts outcomes and treatment responses in pancreatic adenocarcinoma

Authors - Qixian Liu
View Article