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TNS3 Polyclonal antibody

TNS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20053-1-AP

Synonyms

H_NH049I23.2, TEM6, TENS1, tensin 3, TNS3, TPP, Tumor endothelial marker 6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using TNS3 Polyclonal antibody (20053-1-AP)

    WB analysis of mouse kidney using 20053-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20053-1-AP (TNS3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using TNS3 Polyclonal antibody (20053-1-AP)

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using TNS3 Polyclonal antibody (20053-1-AP)

    IHC staining of mouse embryo using 20053-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20053-1-AP (TNS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20053-1-AP targets TNS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tensin 3
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 129 kDa
GenBank Accession NumberNM_022748
Gene Symbol TNS3
Gene ID (NCBI) 64759
RRIDAB_10667405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNS3, also named as TEM6, TENS1 and TPP, may play a role in actin remodeling. It is involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. TNS3 seems to be involved in mammary cell migration. It may be involved in cell migration and bone development. The antibody recognizes all the isoforms of TNS3.

Protocols

Product Specific Protocols
WB protocol for TNS3 antibody 20053-1-APDownload protocol
IHC protocol for TNS3 antibody 20053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Toxicol

EIF4A3-mediated oncogenic circRNA hsa_circ_0001165 advances esophageal squamous cell carcinoma progression through the miR-381-3p/TNS3 pathway

Authors - Xun Zhang
View Article