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  • Featured Product
  • KD/KO Validated

ACK1 Polyclonal antibody

ACK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14304-1-AP

Synonyms

ACK, ACK 1, ACK1, Activated CDC42 kinase 1, p21cdc42Hs, TNK2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A549 using 14304-1-AP

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of SH-SY5Y using 14304-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HT-1080 using 14304-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of A431 using 14304-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of mouse brain using 14304-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HeLa using 14304-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ACK1 Polyclonal antibody (14304-1-AP)

    WB analysis of HepG2 using 14304-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of human breast cancer using 14304-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ACK1 Polyclonal antibody (14304-1-AP)

    IHC staining of human breast cancer using 14304-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inSH-SY5Y cells, HepG2 cells, HT-1080 cells, A431 cells, mouse brain tissue, HeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14304-1-AP targets ACK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACK1 fusion protein Ag5419
Full Name tyrosine kinase, non-receptor, 2
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC028164
Gene Symbol TNK2
Gene ID (NCBI) 10188
RRIDAB_11041713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.

Protocols

Product Specific Protocols
WB protocol for ACK1 antibody 14304-1-APDownload protocol
IHC protocol for ACK1 antibody 14304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Microbiol

The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.

Authors - Lei He
View Article
mouseWB

Biochim Biophys Acta Mol Cell Res

Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport

Authors - G F Sengül
View Article