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CD30/TNFRSF8 Polyclonal antibody

CD30/TNFRSF8 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55132-1-AP

Synonyms

CD30, Ki-1 antigen, Ki 1 antigen, Ki 1, D1S166E

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD30 Polyclonal antibody (55132-1-AP)

    IHC staining of human lymphoma using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD30 Polyclonal antibody (55132-1-AP)

    IHC staining of human lymphoma using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD30 Polyclonal antibody (55132-1-AP)

    IHC staining of human tonsillitis using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55132-1-AP (CD30 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD30 Polyclonal antibody (55132-1-AP)

    IHC staining of human tonsillitis using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55132-1-AP (CD30 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CD30/TNFRSF8 Polyclonal antibody (55132-1-AP)

    IF Staining of human lymphoma using 55132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using CD30/TNFRSF8 Polyclonal antibody (55132-1-AP)

    IF Staining of human lymphoma using 55132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lymphoma tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55132-1-AP targets CD30/TNFRSF8 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated Molecular Weight 64 kDa
GenBank Accession NumberNM_001243
Gene Symbol CD30
Gene ID (NCBI) 943
RRIDAB_2881274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

Protocols

Product Specific Protocols
IHC protocol for CD30/TNFRSF8 antibody 55132-1-APDownload protocol
IF protocol for CD30/TNFRSF8 antibody 55132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols