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  • KD/KO Validated

TM9SF4 Polyclonal antibody

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25595-1-AP

Synonyms

KIAA0255, TM9SF4



Tested Applications

Positive WB detected inA375 cells, A431 cells, HepG2 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TM9SF4 fusion protein Ag22335
Full Name transmembrane 9 superfamily protein member 4
Calculated Molecular Weight 642 aa, 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC021107
Gene Symbol TM9SF4
Gene ID (NCBI) 9777
RRIDAB_2880149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TM9SF4 antibody 25595-1-APDownload protocol
IHC protocol for TM9SF4 antibody 25595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncogene

Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.

Authors - Yifei Zhu
  • KD Validated
mouseWB,IF

Stem Cell Res Ther

TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes

Authors - Libo Yu
  • KO Validated
humanIHC

J Exp Clin Cancer Res

CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells.

Authors - Ying Yu
mouse,humanWB,IHC,IF

Cell Mol Gastroenterol Hepatol

TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease

Authors - Mingxu Xie
  • KD Validated
human,mouseWB,IHC

Nat Commun

TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis

Authors - Zhaoyue Meng
  • KD Validated