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THOP1 Polyclonal antibody

THOP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11738-1-AP

Synonyms

Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOP1 Polyclonal antibody (11738-1-AP)

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using THOP1 Polyclonal antibody (11738-1-AP)

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using THOP1 Polyclonal antibody (11738-1-AP)

    IP experiment of human placenta using 11738-1-AP

    IP result of anti-THOP1 (IP:11738-1-AP, 4ug; Detection:11738-1-AP 1:500) with human placenta tissue lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using THOP1 Polyclonal antibody (11738-1-AP)

    IHC staining of human colon cancer using 11738-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11738-1-AP (THOP1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11738-1-AP targets THOP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOP1 fusion protein Ag2268
Full Name thimet oligopeptidase 1
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC000135
Gene Symbol THOP1
Gene ID (NCBI) 7064
RRIDAB_2303269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

Protocols

Product Specific Protocols
WB protocol for THOP1 antibody 11738-1-APDownload protocol
IHC protocol for THOP1 antibody 11738-1-APDownload protocol
IP protocol for THOP1 antibody 11738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols