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THOC2 Polyclonal antibody
THOC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55178-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
Product Information
55178-1-AP targets THOC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | THO complex 2 |
Calculated Molecular Weight | 183 kDa |
Observed Molecular Weight | 150 kDa |
GenBank Accession Number | NM_020449 |
Gene Symbol | THOC2 |
Gene ID (NCBI) | 57187 |
RRID | AB_2881283 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.
Protocols
Product Specific Protocols | |
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WB protocol for THOC2 antibody 55178-1-AP | Download protocol |
IF protocol for THOC2 antibody 55178-1-AP | Download protocol |
IP protocol for THOC2 antibody 55178-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mol Genet Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability. | ||
Viruses Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.
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