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  • Featured Product
  • KD/KO Validated

THAP1 Polyclonal antibody

THAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12584-1-AP

Synonyms

THAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HepG2 using 12584-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of A549 using 12584-1-AP

    WB result of THAP1 antibody (12584-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP1 transfected A549 cells.

  • Western Blot (WB) analysis of mouse brain tissue using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of mouse brain using 12584-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of mouse heart using 12584-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HeLa using 12584-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HEK-293 using 12584-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HeLa using 12584-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of human heart using 12584-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of A375 using 12584-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of SGC-7901 using 12584-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HEK-293 using 12584-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of human brain using 12584-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HEK-293 using 12584-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THAP1 Polyclonal antibody (12584-1-AP)

    WB analysis of HeLa using 12584-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using THAP1 Polyclonal antibody (12584-1-AP)

    IHC staining of human colon cancer using 12584-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12584-1-AP (THAP1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, A375 cells, SGC-7901 cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12584-1-AP targets THAP1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THAP1 fusion protein Ag3285
Full Name THAP domain containing, apoptosis associated protein 1
Calculated Molecular Weight 213 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC021721
Gene Symbol THAP1
Gene ID (NCBI) 55145
RRIDAB_2201672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.

Protocols

Product Specific Protocols
WB protocol for THAP1 antibody 12584-1-APDownload protocol
IHC protocol for THAP1 antibody 12584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Genet

Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia.

Authors - Esther Meyer
View Article
mouseWB,ChIP

Mol Cell

The dystonia gene THAP1 controls DNA double-strand break repair choice.

Authors - Kenta Shinoda
  • KO Validated
View Article
humanWB

Brain

DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family.

Authors - Fubo Cheng
  • KO Validated
View Article
mouseWB

Ann Neurol

Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.

Authors - Gavarini Sophie S
View Article
humanWB

Ann Neurol

The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6).

Authors - Kaiser Frank J FJ
View Article
mouseWB

Dev Cell

The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.

Authors - Dhananjay Yellajoshyula
View Article