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  • Featured Product
  • KD/KO Validated

THADA Polyclonal antibody

THADA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12909-1-AP

Synonyms

tRNA (32-2'-O)-methyltransferase regulator THADA, Thyroid adenoma-associated protein, thyroid adenoma associated, KIAA1767, GITA

Validation Data Gallery

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  • Western Blot (WB) analysis of CHO cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of CHO cells using 12909-1-AP

    WB result of 12909-1-AP from Takashi Kobayashi.

  • Western Blot (WB) analysis of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of HeLa using 12909-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    WB analysis of A549 using 12909-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of mouse ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human testis using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human testis using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of human pancreas using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of rat ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using THADA Polyclonal antibody (12909-1-AP)

    IHC staining of rat ovary using 12909-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using THADA Polyclonal antibody (12909-1-AP)

    IF Staining of A549 using 12909-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inA549 cells, CHO cells
Positive IHC detected inhuman pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12909-1-AP targets THADA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THADA fusion protein Ag3881
Full Name thyroid adenoma associated
Calculated Molecular Weight 220 kDa
Observed Molecular Weight 183 kDa
GenBank Accession NumberBC025773
Gene Symbol THADA
Gene ID (NCBI) 63892
RRIDAB_10638448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THADA antibody 12909-1-APDownload protocol
IHC protocol for THADA antibody 12909-1-APDownload protocol
IF protocol for THADA antibody 12909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

J Immunother Cancer

THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.

Authors - Chushu Li
  • KD Validated
View Article