TGN46 Polyclonal antibody
TGN46 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 13573-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cerebellum tissue |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 24 publications below |
Product Information
13573-1-AP targets TGN46 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, monkey, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGN46 fusion protein Ag4470 |
| Full Name | trans-golgi network protein 2 |
| Calculated Molecular Weight | 447 aa, 47 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC028219 |
| Gene Symbol | TGN46 |
| Gene ID (NCBI) | 10618 |
| RRID | AB_10597396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGN46 antibody 13573-1-AP | Download protocol |
| IHC protocol for TGN46 antibody 13573-1-AP | Download protocol |
| IF protocol for TGN46 antibody 13573-1-AP | Download protocol |
| IP protocol for TGN46 antibody 13573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. | ||
Cell Death Differ Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade. | ||
EMBO Rep A conserved ion channel function of STING mediates noncanonical autophagy and cell death | ||
PLoS Pathog Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sammy (Verified Customer) (01-20-2024) | A good antibody for IF and also works for WB.
|
FH Amy (Verified Customer) (08-10-2023) | Nice Golgi staining of HEK293T cells.
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FH Christine (Verified Customer) (01-27-2023) | Detects 2 bands around the 85 kDa marker, a sharp one just below and a more fuzzy one just above.
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FH Thomas (Verified Customer) (09-18-2020) | HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour.
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FH Stephen (Verified Customer) (10-01-2019) | RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA
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