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TFPI Polyclonal antibody

TFPI Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20865-1-AP

Synonyms

TFPI1, TFI, Lipoprotein-associated coagulation inhibitor, LACI, Extrinsic pathway inhibitor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TFPI Polyclonal antibody (20865-1-AP)

    WB analysis of HeLa using 20865-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TFPI Polyclonal antibody (20865-1-AP)

    WB analysis of HepG2 using 20865-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20865-1-AP (TFPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TFPI Polyclonal antibody (20865-1-AP)

    IF Staining of HepG2 using 20865-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (20865-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20865-1-AP targets TFPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFPI fusion protein Ag14913
Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Calculated Molecular Weight251aa,29 kDa; 304aa,35 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC015514
Gene Symbol TFPI
Gene ID (NCBI) 7035
RRIDAB_10693682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Protocols

Product Specific Protocols
WB protocol for TFPI antibody 20865-1-APDownload protocol
IF protocol for TFPI antibody 20865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

ADTRP regulates TFPI expression via transcription factor POU1F1 involved in coronary artery disease.

Authors - Chunyan Luo
View Article
humanIHC

Clin Appl Thromb Hemost

Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.

Authors - Kang An
View Article
humanWB

Thromb Res

Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation

Authors - Xian Li
View Article