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  • Featured Product
  • KD/KO Validated

TEKT1 Polyclonal antibody

TEKT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18968-1-AP

Synonyms

Tektin-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using TEKT1 Polyclonal antibody (18968-1-AP)

    WB analysis of human brain using 18968-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TEKT1 Polyclonal antibody (18968-1-AP)

    WB analysis of human testis using 18968-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using TEKT1 Polyclonal antibody (18968-1-AP)

    WB analysis of rat testis using 18968-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TEKT1 Polyclonal antibody (18968-1-AP)

    IHC staining of human testis using 18968-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TEKT1 Polyclonal antibody (18968-1-AP)

    IHC staining of human testis using 18968-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TEKT1 Polyclonal antibody (18968-1-AP)

    IF Staining of HepG2 using 18968-1-AP

    Immunofluorescent analysis of HepG2 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TEKT1 Polyclonal antibody (18968-1-AP)

    IF Staining of MCF-7 using 18968-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

Tested Applications

Positive WB detected inhuman brain tissue, human testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18968-1-AP targets TEKT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEKT1 fusion protein Ag5173
Full Name tektin 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC014599
Gene Symbol TEKT1
Gene ID (NCBI) 83659
RRIDAB_2203324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TEKT1 antibody 18968-1-APDownload protocol
IHC protocol for TEKT1 antibody 18968-1-APDownload protocol
IF protocol for TEKT1 antibody 18968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Curr Biol

CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Authors - Chao Liu
View Article
mouseWB,IP

Cell Mol Life Sci

Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella

Authors - Xin-Yan Geng
View Article
humanWB,IF

Hum Mol Genet

Functional characterization of tektin-1 in motile cilia and evidence for TEKT1 as a new candidate gene for motile ciliopathies.

Authors - Rebecca Ryan
  • KD Validated
View Article
mouseIF

Dev Dyn

Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects

Authors - Pei-Yi Pan
View Article