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TEAD3 Polyclonal antibody

TEAD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13120-1-AP

Synonyms

TEAD-3, TEAD 3, TEA domain family member 3, ETFR 1, DTEF-1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TEAD3 Polyclonal antibody (13120-1-AP)

    WB analysis using 13120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using TEAD3 Polyclonal antibody (13120-1-AP)

    IHC staining of mouse small intestine using 13120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TEAD3 Polyclonal antibody (13120-1-AP)

    IF Staining of MCF-7 using 13120-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13120-1-AP targets TEAD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEAD3 fusion protein Ag3875
Full Name TEA domain family member 3
Calculated Molecular Weight 435 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC027877
Gene Symbol TEAD3
Gene ID (NCBI) 7005
RRIDAB_2203068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

Protocols

Product Specific Protocols
WB protocol for TEAD3 antibody 13120-1-APDownload protocol
IHC protocol for TEAD3 antibody 13120-1-APDownload protocol
IF protocol for TEAD3 antibody 13120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Thyroid hormone regulates muscle fiber type conversion via miR-133a1.

Authors - Duo Zhang
View Article
mouseWB

Res Sq

Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis

Authors - Yang Zhao
View Article
humanWB

Sci Adv

TEAD4 is a master regulator of high-risk nasopharyngeal carcinoma

Authors - Ya-Qin Wang
View Article
mouseIHC

Adv Sci (Weinh)

SUMOylation of TEAD1 Modulates the Mechanism of Pathological Cardiac Hypertrophy

Authors - Xin Shi
View Article
mouseWB

Nat Commun

Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis

Authors - Yang Zhao
View Article