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TDRD5 Polyclonal antibody

TDRD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25239-1-AP

Synonyms

RP11 427G13.1, TDRD5, tudor domain containing 5, TUDOR3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TDRD5 Polyclonal antibody (25239-1-AP)

    WB analysis of mouse testis using 25239-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25239-1-AP (TDRD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TDRD5 Polyclonal antibody (25239-1-AP)

    IP experiment of mouse testis using 25239-1-AP

    IP result of anti-TDRD5 (IP:25239-1-AP, 5ug; Detection:25239-1-AP 1:300) with mouse testis tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRD5 Polyclonal antibody (25239-1-AP)

    IHC staining of mouse testis using 25239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TDRD5 Polyclonal antibody (25239-1-AP)

    IHC staining of mouse testis using 25239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25239-1-AP targets TDRD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDRD5 fusion protein Ag18896
Full Name tudor domain containing 5
Calculated Molecular Weight 981 aa, 110 kDa
Observed Molecular Weight ~116 kDa
GenBank Accession NumberBC130532
Gene Symbol TDRD5
Gene ID (NCBI) 163589
RRIDAB_2879981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDRD5, also named as Tudor domain-containing protein 5, is a 981 amino acid protein, which contains one Tudor domain and three HTH OST-type domains. TDRD5 localizes in the cytiplasm and belongs to the TDRD5 family. TDRD5 is required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity.

Protocols

Product Specific Protocols
WB protocol for TDRD5 antibody 25239-1-APDownload protocol
IHC protocol for TDRD5 antibody 25239-1-APDownload protocol
IP protocol for TDRD5 antibody 25239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols