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TCP10L Polyclonal antibody

TCP10L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13709-1-AP

Synonyms

TCP10-like, TCP10A-1, T-complex protein 10A-1, T-complex protein 10A homolog 1, PRED77

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TCP10L Polyclonal antibody (13709-1-AP)

    WB analysis of mouse testis using 13709-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TCP10L Polyclonal antibody (13709-1-AP)

    WB analysis of mouse testis using 13709-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TCP10L Polyclonal antibody (13709-1-AP)

    IHC staining of human testis using 13709-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TCP10L Polyclonal antibody (13709-1-AP)

    IHC staining of human testis using 13709-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TCP10L Polyclonal antibody (13709-1-AP)

    IF Staining of HeLa using 13709-1-AP

    Immunofluorescent analysis of Hela cells, using 13709-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TCP10L Polyclonal antibody (13709-1-AP)

    IF Staining of HeLa using 13709-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13709-1-AP (TCP10L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13709-1-AP targets TCP10L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCP10L fusion protein Ag4646
Full Name t-complex 10 (mouse)-like
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC033855
Gene Symbol TCP10L
Gene ID (NCBI) 140290
RRIDAB_2255905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCP10L antibody 13709-1-APDownload protocol
IHC protocol for TCP10L antibody 13709-1-APDownload protocol
IF protocol for TCP10L antibody 13709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols