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  • Featured Product
  • KD/KO Validated

TAX1BP1 Polyclonal antibody

TAX1BP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14424-1-AP

Synonyms

TRAF6-binding protein, Tax1-binding protein 1, T6BP, PRO0105

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAX1BP1 Polyclonal antibody (14424-1-AP)

    WB analysis using 14424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14424-1-AP (TAX1BP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TAX1BP1 Polyclonal antibody (14424-1-AP)

    IHC staining of human skin cancer using 14424-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14424-1-AP (TAX1BP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAX1BP1 Polyclonal antibody (14424-1-AP)

    IF Staining of HeLa using 14424-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TAX1BP1 antibody (14424-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14424-1-AP targets TAX1BP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAX1BP1 fusion protein Ag5789
Full Name Tax1 (human T-cell leukemia virus type I) binding protein 1
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC050358
Gene Symbol TAX1BP1
Gene ID (NCBI) 8887
RRIDAB_2198921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAX1BP1 (also named as T6BP and TXBP151) inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. It is degraded by caspase-3-like family proteins upon TNF-induced apoptosis. TAX1BP1 may also play a role in the pro-inflammatory cytokine IL-1 signaling cascade. TAX1BP1 binds TRAF6 but not TRAFs 1-5.

Protocols

Product Specific Protocols
WB protocol for TAX1BP1 antibody 14424-1-APDownload protocol
IHC protocol for TAX1BP1 antibody 14424-1-APDownload protocol
IF protocol for TAX1BP1 antibody 14424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

Authors - Zhenyu Zhong
View Article
WB

Mol Cell

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

Authors - Shouqiao Hou
View Article
mouseWB

Redox Biol

A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.

Authors - Qifeng Deng
View Article
mouseWB

Autophagy

TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy.

Authors - Wenxian Wu
View Article
humanIF

Autophagy

Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.

Authors - Belinda S Hall
View Article
humanIF

Dev Cell

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Authors - Maria Zachari
View Article