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SURF2 Polyclonal antibody

SURF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16522-1-AP

Synonyms

surfeit 2, Surf-2, SURF 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SURF2 Polyclonal antibody (16522-1-AP)

    WB analysis using 16522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using SURF2 Polyclonal antibody (16522-1-AP)

    WB analysis of BxPC-3 using 16522-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SURF2 Polyclonal antibody (16522-1-AP)

    WB analysis of COLO 320 using 16522-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SURF2 Polyclonal antibody (16522-1-AP)

    WB analysis of HeLa using 16522-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SURF2 Polyclonal antibody (16522-1-AP)

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SURF2 Polyclonal antibody (16522-1-AP)

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SURF2 Polyclonal antibody (16522-1-AP)

    IF Staining of HeLa using 16522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16522-1-AP (SURF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SURF2 Polyclonal antibody (16522-1-AP)

    IF Staining of HeLa using 16522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16522-1-AP (SURF2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16522-1-AP targets SURF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SURF2 fusion protein Ag9745
Full Name surfeit 2
Calculated Molecular Weight 256 aa, 30 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014411
Gene Symbol SURF2
Gene ID (NCBI) 6835
RRIDAB_2255415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SURF2 antibody 16522-1-APDownload protocol
IHC protocol for SURF2 antibody 16522-1-APDownload protocol
IF protocol for SURF2 antibody 16522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols