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  • Featured Product
  • KD/KO Validated

SURF1 Polyclonal antibody

SURF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15379-1-AP

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SURF1 Polyclonal antibody (15379-1-AP)

    WB analysis using 15379-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SURF1 Polyclonal antibody (15379-1-AP)

    IP experiment of HeLa using 15379-1-AP

    IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using SURF1 Polyclonal antibody (15379-1-AP)

    IHC staining of human colon using 15379-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SURF1 Polyclonal antibody (15379-1-AP)

    IHC staining of human colon using 15379-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SURF1 Polyclonal antibody (15379-1-AP)

    IF Staining of HeLa using 15379-1-AP

    Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15379-1-AP targets SURF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SURF1 fusion protein Ag3959
Full Name surfeit 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-31 kDa
GenBank Accession NumberBC028314
Gene Symbol SURF1
Gene ID (NCBI) 6834
RRIDAB_2239968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SURF1 antibody 15379-1-APDownload protocol
IHC protocol for SURF1 antibody 15379-1-APDownload protocol
IF protocol for SURF1 antibody 15379-1-APDownload protocol
IP protocol for SURF1 antibody 15379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

SURF1 mutations in Chinese patients with Leigh syndrome: Novel mutations, mutation spectrum, and the functional consequences.

Authors - Yuanyuan Li
  • KO Validated
View Article
humanWB

Cell Rep

Mitochondrial respiration restricts Listeria monocytogenes infection by slowing down host cell receptor recycling.

Authors - Anna Spier
  • KD Validated
View Article