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STOML2 Polyclonal antibody

STOML2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10348-1-AP

Synonyms

SLP2, SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2



Tested Applications

Positive WB detected inmouse brain tissue, C6 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human endometrial cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10348-1-AP targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STOML2 fusion protein Ag0363
Full Name stomatin (EPB72)-like 2
Calculated Molecular Weight 356 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC002442
Gene Symbol STOML2
Gene ID (NCBI) 30968
RRIDAB_2286822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for STOML2 antibody 10348-1-APDownload protocol
IHC protocol for STOML2 antibody 10348-1-APDownload protocol
IF protocol for STOML2 antibody 10348-1-APDownload protocol
IP protocol for STOML2 antibody 10348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
humanWB,IHC

Clin Cancer Res

Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma.

Authors - Zhang Liyong L
humanWB

EMBO Rep

The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L.

Authors - Timothy Wai
humanWB

Hum Mol Genet

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Authors - McCray Brett A BA
humanWB

J Pathol

Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway.

Authors - Song Libing L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Gaurav (Verified Customer) (02-15-2024)

Good antibody which works as per the description. Detects the endogenous proteins without any problem.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293, Smooth Muscle Cells
STOML2 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in HEK293, Smooth Muscle Cells (Cat no:10348-1-AP)
FH

Hussein (Verified Customer) (03-23-2021)

I validated the antibody specificity using different siRNAs. The signal is strong in IB and bright in IF and IHC.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: WB 1:1000 (each dilution was used 3 times); IP 1:200; IHC 1:400
  • Cell Tissue Type: Breast cancer cell lines: MDA-MB-231, MDA-MB-361, BT-474, JIMT-1
FH

SITING (Verified Customer) (08-31-2020)

excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: heart
STOML2 Antibody Western Blot validation (1:1000 dilution) in heart (Cat no:10348-1-AP)