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Stomatin Polyclonal antibody

Stomatin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12046-1-AP

Synonyms

STOM, Erythrocyte membrane protein band 7.2, EPB72, EPB7, BND7

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Stomatin Polyclonal antibody (12046-1-AP)

    WB analysis of HeLa using 12046-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Stomatin Polyclonal antibody (12046-1-AP)

    WB analysis of human liver using 12046-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using Stomatin Polyclonal antibody (12046-1-AP)

    WB analysis of human lung using 12046-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Stomatin Polyclonal antibody (12046-1-AP)

    WB analysis of K-562 using 12046-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (STOM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Stomatin Polyclonal antibody (12046-1-AP)

    WB analysis of A549 using 12046-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12046-1-AP (Stomatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Stomatin Polyclonal antibody (12046-1-AP)

    IP experiment of HeLa using 12046-1-AP

    IP result of anti-Stomatin (IP:12046-1-AP, 3ug; Detection:12046-1-AP 1:300) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Stomatin Polyclonal antibody (12046-1-AP)

    IHC staining of human lung cancer using 12046-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Stomatin Polyclonal antibody (12046-1-AP)

    IHC staining of human lung cancer using 12046-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12046-1-AP (Stomatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Stomatin Polyclonal antibody (12046-1-AP)

    IF Staining of A549 using 12046-1-AP

    Immunofluorescent analysis of A549 cells using 12046-1-AP (STOM antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, human liver tissue, human lung tissue, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12046-1-AP targets Stomatin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Stomatin fusion protein Ag2676
Full Name stomatin
Calculated Molecular Weight 288 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC010703
Gene Symbol Stomatin
Gene ID (NCBI) 2040
RRIDAB_2197406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

Protocols

Product Specific Protocols
WB protocol for Stomatin antibody 12046-1-APDownload protocol
IHC protocol for Stomatin antibody 12046-1-APDownload protocol
IF protocol for Stomatin antibody 12046-1-APDownload protocol
IP protocol for Stomatin antibody 12046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Med

Facile discovery of red blood cell deformation and compromised membrane/skeleton assembly in Prader-Willi syndrome

Authors - Yashuang Yang
View Article
humanWB

Vox Sang

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools.

Authors - Rubin O O
View Article
humanIHC

Chin J Cancer Res

Stomatin plays a suppressor role in non-small cell lung cancer metastasis.

Authors - Huaying An
View Article
humanWB,CoIP

Biophys J

Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains

Authors - Artem G Ayuyan
View Article