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STARD8 Polyclonal antibody

STARD8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13899-1-AP

Synonyms

START domain-containing protein 8, StAR-related lipid transfer protein 8, KIAA0189, DLC-3, DLC3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human placenta using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13899-1-AP (STARD8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human placenta using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13899-1-AP (STARD8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human stomach cancer using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13899-1-AP (STARD8 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human stomach cancer using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13899-1-AP (STARD8 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human kidney using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13899-1-AP (STARD8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using STARD8 Polyclonal antibody (13899-1-AP)

    IHC staining of human kidney using 13899-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13899-1-AP (STARD8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STARD8 Polyclonal antibody (13899-1-AP)

    IF Staining of HEK-293 using 13899-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 13899-1-AP (STARD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman placenta tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13899-1-AP targets STARD8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STARD8 fusion protein Ag4865
Full Name StAR-related lipid transfer (START) domain containing 8
Calculated Molecular Weight 1023 aa, 113 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC035587
Gene Symbol STARD8
Gene ID (NCBI) 9754
RRIDAB_2197826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for STARD8 antibody 13899-1-APDownload protocol
IF protocol for STARD8 antibody 13899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Cell Biol

CDK5 is a major regulator of the tumor suppressor DLC1.

Authors - Brajendra K Tripathi
View Article
mouseWB

Aging (Albany NY)

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells.

Authors - Jie Liang
View Article
humanWB,IHC

Mol Med Rep

High expression of DLC family proteins predicts better prognosis and inhibits tumor progression in NSCLC.

Authors - Li Sun
View Article
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
View Article