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STARD10 Polyclonal antibody

STARD10 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17048-1-AP

Synonyms

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STARD10 Polyclonal antibody (17048-1-AP)

    WB analysis using 17048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17048-1-AP (STARD10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using STARD10 Polyclonal antibody (17048-1-AP)

    IP experiment of HepG2 using 17048-1-AP

    IP result of anti-STARD10 (IP:17048-1-AP, 4ug; Detection:17048-1-AP 1:300) with HepG2 cells lysate 3400 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using STARD10 Polyclonal antibody (17048-1-AP)

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using STARD10 Polyclonal antibody (17048-1-AP)

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17048-1-AP targets STARD10 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STARD10 fusion protein Ag10705
Full Name StAR-related lipid transfer (START) domain containing 10
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC007919
Gene Symbol STARD10
Gene ID (NCBI) 10809
RRIDAB_2255485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

Protocols

Product Specific Protocols
WB protocol for STARD10 antibody 17048-1-APDownload protocol
IHC protocol for STARD10 antibody 17048-1-APDownload protocol
IP protocol for STARD10 antibody 17048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols