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ST7 Polyclonal antibody

ST7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11945-1-AP

Synonyms

ETS7q, FAM4A, FAM4A1, HELG, Protein FAM4A1, Protein HELG, RAY1, SEN4, ST7, TSG7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST7 Polyclonal antibody (11945-1-AP)

    WB analysis using 11945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ST7 Polyclonal antibody (11945-1-AP)

    WB analysis of mouse brain using 11945-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human liver cancer using 11945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human liver cancer using 11945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human pancreas using 11945-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ST7 Polyclonal antibody (11945-1-AP)

    IHC staining of human pancreas using 11945-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 11945-1-AP (ST7 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, Jurkat cells, PC-3 cells
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11945-1-AP targets ST7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST7 fusion protein Ag2547
Full Name suppression of tumorigenicity 7
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC030954
Gene Symbol ST7
Gene ID (NCBI) 7982
RRIDAB_2270936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region on chromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor gene with highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane protein belonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent result suggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining the cellular structure of the cell and involved in oncogenic pathways.

Protocols

Product Specific Protocols
WB protocol for ST7 antibody 11945-1-APDownload protocol
IHC protocol for ST7 antibody 11945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Genetic validation of the protein arginine methyltransferase PRMT5 as a candidate therapeutic target in glioblastoma.

Authors - Fengting Yan
View Article