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TRAPA/SSR1 Polyclonal antibody

TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10583-1-AP

Synonyms

SSR1, TRAP alpha, TRAP a, Translocon-associated protein subunit alpha, SSR-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    WB analysis using 10583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    WB analysis using 10583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    WB analysis of human liver using 10583-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    WB analysis of mouse liver using 10583-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    WB analysis using 10583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    IHC staining of human ovary tumor using 10583-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    IHC staining of human ovary tumor using 10583-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    IHC staining of human lung using 10583-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    IHC staining of human lung using 10583-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TRAPA/SSR1 Polyclonal antibody (10583-1-AP)

    IF Staining of HEK-293 using 10583-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TRAPA/SSR1 antibody (10583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inCOS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells
Positive IHC detected inhuman ovary tumor tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPA/SSR1 fusion protein Ag0878
Full Name signal sequence receptor, alpha
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007710
Gene Symbol TRAPA
Gene ID (NCBI) 6745
RRIDAB_2195616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Protocols

Product Specific Protocols
WB protocol for TRAPA/SSR1 antibody 10583-1-APDownload protocol
IHC protocol for TRAPA/SSR1 antibody 10583-1-APDownload protocol
IF protocol for TRAPA/SSR1 antibody 10583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
View Article
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
View Article
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
View Article
humanIF

Biosens Bioelectron

Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.

Authors - Mao Chen
View Article
humanWB

FASEB J

GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin.

Authors - S Orvokki Mattila
View Article
ratWB,IF

Exp Cell Res

Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers.

Authors - Kaakinen Mika M
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Chatchai (Verified Customer) (09-26-2019)

This anti-SSR1 is very good, presents the specific band at the right size of the protein.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:500 for WB
  • Cell Tissue Type: A549, HEK-293