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- KD/KO Validated
SSBP1 Polyclonal antibody
SSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 12212-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, A375 cells, mouse pancreas tissue, rat pancreas tissue, Caco-2 cells |
| Positive IHC detected in | human stomach cancer tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 24 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
12212-1-AP targets SSBP1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SSBP1 fusion protein Ag2860 |
| Full Name | single-stranded DNA binding protein 1 |
| Calculated Molecular Weight | 148 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC000895 |
| Gene Symbol | SSBP1 |
| Gene ID (NCBI) | 6742 |
| RRID | AB_2195320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| IHC protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| IF protocol for SSBP1 antibody 12212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Mol Cell Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. | ||
Sci Adv The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
| ||
Nucleic Acids Res Top3α is the replicative topoisomerase in mitochondrial DNA replication. |
