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SPRY2 Polyclonal antibody
SPRY2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11383-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SW 1990 cells, Jurkat cells, A375 cells, Neuro-2a cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
11383-1-AP targets SPRY2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPRY2 fusion protein Ag1918 |
| Full Name | sprouty homolog 2 (Drosophila) |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35-40 kDa, 60~65 kDa |
| GenBank Accession Number | BC015745 |
| Gene Symbol | SPRY2 |
| Gene ID (NCBI) | 10253 |
| RRID | AB_2195287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs). In addition to its well-reported function as an inhibitor of the RAS/RAF/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K/AKT signalling pathway through PTEN, and inhibits ERK/MAPK activity via EGFR trafficking. SPRY2 was detected 60-65 kDa (PMID:29291435).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| IHC protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| IF protocol for SPRY2 antibody 11383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Crohns Colitis MSCs-exosomes can promote macrophage M2 polarization via exosomal miR-21-5p through Mesenteric injection: a promising way to attenuate murine colitis | ||
Int J Mol Sci Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway. | ||
J Cell Mol Med Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.
| ||
Toxicol Appl Pharmacol Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways. | ||
Cell Biol Int Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways. | ||
Breast Cancer Res Loss of SPRY2 contributes to cancer-associated fibroblasts activation and promotes breast cancer development
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Christine (Verified Customer) (01-27-2023) | Detects two bands on HEK293T lysates. main one between 36 and 48 kDa, second one between 48 and 85 kDa, in accordance with datasheet
|
FH Silvia (Verified Customer) (01-31-2022) | The antibody worked really well and gave me a clear band on Western Blot around 50 kDa.
|
