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SPRR1B Polyclonal antibody

SPRR1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 11959-1-AP

Synonyms

14.9 kDa pancornulin, CORNIFIN, Cornifin B, GADD33, Small proline rich protein IB, SPR IB, SPRR1, SPRR1B

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin tissue using SPRR1B Polyclonal antibody (11959-1-AP)

    IHC staining of human skin using 11959-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SPRR1B Polyclonal antibody (11959-1-AP)

    IHC staining of human skin using 11959-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SPRR1B Polyclonal antibody (11959-1-AP)

    IHC staining of human skin using 11959-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SPRR1B Polyclonal antibody (11959-1-AP)

    IHC staining of human skin using 11959-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

11959-1-AP targets SPRR1B in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPRR1B fusion protein Ag2563
Full Name small proline-rich protein 1B (cornifin)
Calculated Molecular Weight 89 aa, 10 kDa
GenBank Accession NumberBC056240
Gene Symbol SPRR1B
Gene ID (NCBI) 6699
RRIDAB_2877807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This protein functions as a component of the cross-linked envelope in squamous differentiating cells. It runs as a 15-kDa protein in unreducing SDS PAGE, whereas under reducing conditions it runs as 23 kDa one.

Protocols

Product Specific Protocols
IHC protocol for SPRR1B antibody 11959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Discov

Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis.

Authors - Shengqian Dou
View Article
humanIHC

Front Oncol

Long Noncoding RNA HAGLROS Promotes the Malignant Progression of Bladder Cancer by Regulating the miR-330-5p/SPRR1B Axis.

Authors - Shiwei Xiao
View Article