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CD43 Polyclonal antibody

CD43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13959-1-AP

Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CD43 Polyclonal antibody (13959-1-AP)

    WB analysis of K-562 using 13959-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13959-1-AP (CD43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD43 Polyclonal antibody (13959-1-AP)

    WB analysis of Jurkat using 13959-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13959-1-AP (CD43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD43 Polyclonal antibody (13959-1-AP)

    IHC staining of human lymphoma using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13959-1-AP (CD43 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD43 Polyclonal antibody (13959-1-AP)

    IHC staining of human lymphoma using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13959-1-AP (CD43 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13959-1-AP targets CD43 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD43 fusion protein Ag4836
Full Name sialophorin
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC012350
Gene Symbol CD43
Gene ID (NCBI) 6693
RRIDAB_10694668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protocols

Product Specific Protocols
WB protocol for CD43 antibody 13959-1-APDownload protocol
IHC protocol for CD43 antibody 13959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols