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SPERT Polyclonal antibody

SPERT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17826-1-AP

Synonyms

CBY2, NURIT, Protein chibby homolog 2, spermatid associated, Spermatid associated protein, SPERT

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of mouse testis using 17826-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of PC-3 using 17826-1-AP

    WB result of SPERT antibody (17826-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPERT transfected PC-3 cells.

  • Western Blot (WB) analysis of mouse spermatophore tissue using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of mouse spermatophore using 17826-1-AP

    mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of PC-3 using 17826-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    IF Staining of PC-3 using 17826-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, mouse spermatophore tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17826-1-AP targets SPERT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPERT fusion protein Ag12200
Full Name spermatid associated
Calculated Molecular Weight 448 aa, 47 kDa, 52 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC027609
Gene Symbol SPERT
Gene ID (NCBI) 220082
RRIDAB_2195674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

Protocols

Product Specific Protocols
WB protocol for SPERT antibody 17826-1-APDownload protocol
IF protocol for SPERT antibody 17826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Reprod Biol

Identification of a novel mutation in chibby family member 2 in a non-obstructive azoospermic patient

Authors - Guohui Zhang
View Article