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SOX2 Monoclonal antibody
SOX2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
4B9B10
Cat no : 66411-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U-251 cells, HEK-293 cells, MCF-7 cells, fetal human brain tissue, pig brain tissue, C6 cells |
| Positive IHC detected in | human lung cancer tissue, mouse brain tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 32 publications below |
| IHC | See 1 publications below |
| IF | See 13 publications below |
Product Information
66411-1-Ig targets SOX2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SOX2 fusion protein Ag13635 |
| Full Name | SRY (sex determining region Y)-box 2 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC013923 |
| Gene Symbol | SOX2 |
| Gene ID (NCBI) | 6657 |
| RRID | AB_2881783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Background
SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.
What is the molecular weight of Sox2?
34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.
What is the tissue specificity of Sox2?
In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).
In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).
What is the role of Sox2 in pluripotency?
Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).
What is the role of Sox2 in cancer?
The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX2 antibody 66411-1-Ig | Download protocol |
| IHC protocol for SOX2 antibody 66411-1-Ig | Download protocol |
| IF protocol for SOX2 antibody 66411-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Bone Res Type II collagen-positive progenitors are important stem cells in controlling skeletal development and vascular formation. | ||
Nanoscale An assembly-inducing PDC enabling the efficient nuclear delivery of nucleic acid for cancer stem-like cell suppression | ||
Oncogene MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer. | ||
Int J Mol Sci Addressing Doxorubicin Resistance in Bone Sarcomas Using Novel Drug-Resistant Models. | ||
iScience Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
