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  • Featured Product
  • KD/KO Validated

SNX6 Polyclonal antibody

SNX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10114-1-AP

Synonyms

TRAF4-associated factor 2, Sorting nexin-6, N-terminally processed, Sorting nexin-6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX6 Polyclonal antibody (10114-1-AP)

    WB analysis using 10114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SNX6 Polyclonal antibody (10114-1-AP)

    WB analysis of human placenta using 10114-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNX6 Polyclonal antibody (10114-1-AP)

    WB analysis of mouse brain using 10114-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SNX6 Polyclonal antibody (10114-1-AP)

    WB analysis of HepG2 using 10114-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SNX6 Polyclonal antibody (10114-1-AP)

    WB analysis of HepG2 using 10114-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SNX6 Polyclonal antibody (10114-1-AP)

    IP experiment of HepG2 using 10114-1-AP

    IP result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of mouse brain using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of mouse brain using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human prostate cancer using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human prostate cancer using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human prostate cancer using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human prostate cancer using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human heart using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SNX6 Polyclonal antibody (10114-1-AP)

    IHC staining of human heart using 10114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SNX6 Polyclonal antibody (10114-1-AP)

    IF Staining of HepG2 using 10114-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10114-1-AP targets SNX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX6 fusion protein Ag0168
Full Name sorting nexin 6
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC001798
Gene Symbol SNX6
Gene ID (NCBI) 58533
RRIDAB_2192720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants

Protocols

Product Specific Protocols
WB protocol for SNX6 antibody 10114-1-APDownload protocol
IHC protocol for SNX6 antibody 10114-1-APDownload protocol
IF protocol for SNX6 antibody 10114-1-APDownload protocol
IP protocol for SNX6 antibody 10114-1-APDownload protocol
FC protocol for SNX6 antibody 10114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
View Article
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.

Authors - Pengfei Hu
  • KD Validated
View Article
humanWB

Front Immunol

Zika virus modulates mitochondrial dynamics, mitophagy, and mitochondria-derived vesicles to facilitate viral replication in trophoblast cells

Authors - Jae Kyung Lee
View Article