Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of HeLa using 60154-2-Ig
WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells.
WB analysis using 60154-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 60154-2-Ig
A375 cells were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 60154-2-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 60154-2-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
FC experiment of Jurkat using 60154-2-Ig
1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig, Clone:3C10B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

SMN (Human,Mouse,Rat) Monoclonal antibody

SMN (Human,Mouse,Rat) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 60154-2-Ig

Clone No.3C10B5

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

SMN, Gemin-1, Gemin 1, Component of gems 1, C BCD541

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, A375 cells, HeLa cells, T-47D cells, HEK-293 cells, MOLT-4 cells, Jurkat cells, Raji cells
Positive IHC detected in	human rectal cancer tissue, human colon cancer, mouse brain tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60154-2-Ig targets SMN (Human,Mouse,Rat) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	SMN (Human,Mouse,Rat) fusion protein Ag14333 Predict reactive species
Full Name	survival of motor neuron 2, centromeric
Calculated Molecular Weight	282 aa, 30 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC000908
Gene Symbol	SMN
Gene ID (NCBI)	6607
RRID	AB_10792407
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.

Protocols

Product Specific Protocols
WB protocol for SMN (Human,Mouse,Rat) antibody 60154-2-Ig	Download protocol
IHC protocol for SMN (Human,Mouse,Rat) antibody 60154-2-Ig	Download protocol
IF protocol for SMN (Human,Mouse,Rat) antibody 60154-2-Ig	Download protocol