HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12018-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12018-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12018-1-AP
IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)
HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12018-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12018-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human medulloblastoma using 12018-1-AP
Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12018-1-AP (SMARCC2 antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of HeLa using 12018-1-AP
IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.
IF/ICC Figures
IF Staining of HepG2 using 12018-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BAF170 antibody (12018-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
BAF170 Polyclonal antibody
Catalog Number
12018-1-AP
Citations
3
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
