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SMAD5 Polyclonal antibody

SMAD5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12167-1-AP

Synonyms

MADH5, MAD homolog 5, JV5-1, JV5 1, hSmad5

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SMAD5 Polyclonal antibody (12167-1-AP)

    WB analysis of K-562 using 12167-1-AP

    WB result of SMAD5 antibody (12167-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD5 transfected K-562 cells.

  • Western Blot (WB) analysis of various lysates using SMAD5 Polyclonal antibody (12167-1-AP)

    WB analysis using 12167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SMAD5 Polyclonal antibody (12167-1-AP)

    WB analysis of K-562 using 12167-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP (SMAD5 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HeLa cells using SMAD5 Polyclonal antibody (12167-1-AP)

    IP experiment of HeLa using 12167-1-AP

    IP result of anti-SMAD5 (IP:12167-1-AP, 4ug; Detection:12167-1-AP 1:5000) with HeLa cells lysate 1760 ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SMAD5 Polyclonal antibody (12167-1-AP)

    IF Staining of MCF-7 using 12167-1-AP

    Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMAD5 Polyclonal antibody (12167-1-AP)

    IF Staining of HeLa using 12167-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD5 antibody (12167-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMAD5 Polyclonal antibody (12167-1-AP)

    IF Staining of HeLa using 12167-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD5 antibody (12167-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, NIH/3T3 cells, PC-12 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12167-1-AP targets SMAD5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD5 fusion protein Ag2809
Full Name SMAD family member 5
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC009682
Gene Symbol SMAD5
Gene ID (NCBI) 4090
RRIDAB_2286502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Protocols

Product Specific Protocols
WB protocol for SMAD5 antibody 12167-1-APDownload protocol
IF protocol for SMAD5 antibody 12167-1-APDownload protocol
IP protocol for SMAD5 antibody 12167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

PLoS Biol

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Authors - Christian Hiepen
View Article
humanWB

IUBMB Life

LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis

Authors - Shuning Hao
View Article
humanFC

Sci Rep

IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.

Authors - Gina Dörpholz
View Article
ratWB

Int J Biochem Cell Biol

The Role of SIRT6 in the Differentiation of Vascular Smooth Muscle Cells in Response to Cyclic Strain.

Authors - Qing-Ping Yao
View Article
mouseWB

Sci Rep

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Authors - Xueni Wang
View Article
mouseWB

PLoS One

Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.

Authors - Zhang Xing-Yi XY
View Article