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- KD/KO Validated
SMAD3 Polyclonal antibody
SMAD3 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25494-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 67 publications below |
| IHC | See 9 publications below |
| IF | See 3 publications below |
Product Information
25494-1-AP targets SMAD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat, canine, Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | SMAD family member 3 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | NM_005902 |
| Gene Symbol | SMAD3 |
| Gene ID (NCBI) | 4088 |
| RRID | AB_2880102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMAD3 antibody 25494-1-AP | Download protocol |
| IHC protocol for SMAD3 antibody 25494-1-AP | Download protocol |
| IF protocol for SMAD3 antibody 25494-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Circulation Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation. | ||
Cell Death Differ REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway. | ||
Oncogene The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis.
| ||
Stem Cell Reports Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.
| ||
Oxid Med Cell Longev WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling. | ||
Int J Nanomedicine A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway. |
