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  • Featured Product
  • KD/KO Validated

SMAD1 Polyclonal antibody

SMAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10429-1-AP

Synonyms

MADH1, JV4-1, JV41, hSMAD1, BSP-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMAD1 Polyclonal antibody (10429-1-AP)

    WB analysis using 10429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMAD1 Polyclonal antibody (10429-1-AP)

    WB analysis of HEK-293 using 10429-1-AP

    WB result of SMAD1 antibody (10429-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HepG2 cells using SMAD1 Polyclonal antibody (10429-1-AP)

    WB analysis of HepG2 using 10429-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    WB analysis of mouse heart using 10429-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    IP experiment of mouse heart using 10429-1-AP

    IP result of anti-SMAD1 (IP:10429-1-AP, 4ug; Detection:10429-1-AP 1:500) with mouse heart tissue lysate 8000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    IHC staining of human colon using 10429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    IHC staining of human colon using 10429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    IHC staining of rat liver using 10429-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using SMAD1 Polyclonal antibody (10429-1-AP)

    IHC staining of rat liver using 10429-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMAD1 Polyclonal antibody (10429-1-AP)

    IF Staining of HeLa using 10429-1-AP

    Immunofluorescent analysis of HeLa cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMAD1 Polyclonal antibody (10429-1-AP)

    IF Staining of HepG2 using 10429-1-AP

    Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHT-1080 cells, HEK-293 cells, HepG2 cells, mouse heart tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 27 publications below

Product Information

10429-1-AP targets SMAD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMAD1 fusion protein Ag0701
Full Name SMAD family member 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2247334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Protocols

Product Specific Protocols
WB protocol for SMAD1 antibody 10429-1-APDownload protocol
IHC protocol for SMAD1 antibody 10429-1-APDownload protocol
IF protocol for SMAD1 antibody 10429-1-APDownload protocol
IP protocol for SMAD1 antibody 10429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Carbohydr Polym

In vitro and in silico anti-osteoporosis activities and underlying mechanisms of a fructan, ABW90-1, from Achyranthes bidentate.

Authors - Tianyu Li
View Article
mouseWB

Cell Death Discov

TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9

Authors - Huakun Huang
View Article
mouseWB

Front Immunol

Silencing SOCS3 Markedly Deteriorates Spondyloarthritis in Mice Induced by Minicircle DNA Expressing IL23.

Authors - Yuhai Chen
View Article
mouseWB

Front Mol Neurosci

Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.

Authors - Tianda Chen
View Article
humanWB

IUBMB Life

LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis

Authors - Shuning Hao
View Article
mouseWB

Ecotoxicol Environ Saf

Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.

Authors - Di Wang
View Article