• Featured Product
  • KD/KO Validated

CD98/SLC3A2 Polyclonal antibody

CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15193-1-AP

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain



Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IHC detected inrat testis tissue, mouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15193-1-AP targets CD98/SLC3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD98/SLC3A2 fusion protein Ag7280
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight85-94 kDa, 120-130 kDa
GenBank Accession NumberBC001061
Gene Symbol CD98
Gene ID (NCBI) 6520
RRIDAB_2254909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
WB protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
IHC protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
IF protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Nano

Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy

Authors - Rangrang Fan
humanWB,IHC

Sci Transl Med

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.

Authors - Kana Hasegawa
mouseIF

Nat Commun

Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.

Authors - Weiwei Yu
humanIF

J Allergy Clin Immunol

Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.

Authors - Siddhartha S Saha
humanWB

J Hazard Mater

ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.

Authors - Xuan Lai
mouseWB

Cell Rep

Brown adipocyte ATF4 activation improves thermoregulation and systemic metabolism

Authors - Esther Paulo