Featured Product
KD/KO Validated

ZIP8 Polyclonal antibody

Name: ZIP8 Polyclonal antibody
Brand: Proteintech
SKU: 20459-1-AP

ZIP8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20459-1-AP

Synonyms

BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse lung using 20459-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 20459-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 20459-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 20459-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 20459-1-AP
IP result of anti-ZIP8 (IP:20459-1-AP, 4ug; Detection:20459-1-AP 1:500) with mouse liver tissue lysate 6000ug.
IHC staining of human kidney using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 40x lens).
IHC staining of mouse small intestine using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 20459-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 20459-1-AP (ZIP8 antibody) at dilution of 1:100 (under 10x lens).

-/ -

Cat No. 20459-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Proteintech Guarantee

Request bulk or custom quote

Tested Applications

Positive WB detected in	mouse lung tissue, mouse heart tissue, U-937 cells, mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human kidney tissue, human placenta tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 30 publications below
IHC	See 8 publications below
IF	See 10 publications below
FC	See 2 publications below

Product Information

The immunogen of 20459-1-AP is ZIP8 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ZIP8 fusion protein Ag14292
Full Name	solute carrier family 39 (zinc transporter), member 8
Calculated Molecular Weight	460 aa, 50 kDa
Observed Molecular Weight	42-46 kDa, 75-90 kDa
GenBank Accession Number	BC012125
Gene Symbol	ZIP8
Gene ID (NCBI)	64116
RRID	AB_10697830
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.

Protocols

Product Specific Protocols
WB protocol for ZIP8 antibody 20459-1-AP	Download protocol
IHC protocol for ZIP8 antibody 20459-1-AP	Download protocol
IP protocol for ZIP8 antibody 20459-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cell Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis. Authors - Jin-Hong Kim View Article
mouse	IF	Nat Commun The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease Authors - Eun-Kyung Choi View Article
mouse	WB,IF	J Clin Invest Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity. Authors - Wen Lin KO Validated View Article
mouse	WB	Acta Neuropathol Commun Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder. Authors - Alexandra Grubman View Article
rat	WB	Int J Mol Sci Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes. Authors - Yusuf Olgar View Article
mouse	WB,IF	Int Immunopharmacol ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx. Authors - Gong Cheng KD Validated View Article

ZIP8 Polyclonal antibody

ZIP8 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse lung using 20459-1-AP

WB analysis of U-937 using 20459-1-AP

WB analysis of mouse heart using 20459-1-AP

WB analysis of mouse liver using 20459-1-AP

IP experiment of mouse liver using 20459-1-AP

IHC staining of human kidney using 20459-1-AP

IHC staining of human kidney using 20459-1-AP

IHC staining of human kidney using 20459-1-AP

IHC staining of mouse small intestine using 20459-1-AP

IHC staining of mouse small intestine using 20459-1-AP

IHC staining of mouse small intestine using 20459-1-AP

IHC staining of mouse kidney using 20459-1-AP

IHC staining of mouse kidney using 20459-1-AP

IHC staining of mouse small intestine using 20459-1-AP

IHC staining of mouse lung using 20459-1-AP

IHC staining of mouse lung using 20459-1-AP

IHC staining of human placenta using 20459-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis.

The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease

Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes.

ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.